| Character | Actor | First Episode | Last Episode | |
 | Dr Jack Kerruish | Kevin Whately | Series 1 Episode 1 | Series 3 Episode 15 |
 | Dr Beth Glover | Amanda Burton | Series 1 Episode 1 | Series 3 Episode 15 |
 | Dr Will Preston | Simon Shepherd | Series 1 Episode 1 | Series 12 Episode 13 |
 | Isabel de Gines | Sylvia Syms | Series 1 Episode 1 | Series 3 Episode 3 |
 | Sarah Preston | Jacqueline Leonard | Series 1 Episode 2 | Series 4 Episode 1 |
 | Kim Beardsmore | Esther Coles | Series 1 Episode 1 | Series 7 Episode 2 |
 | Alice North | Margery Mason | Series 1 Episode 1 | Series 12 Episode 13 |
 | Chloe White | Hazel Ellerby | Series 1 Episode 1 | Series 6 Episode 14 |
 | James White | Richard Platt | Series 1 Episode 1 | Series 6 Episode 14 |
 | Ellie Ndebala | Sharon Hinds | Series 1 Episode 1 | Series 2 Episode 13 |
 | Trevor Sharp | Shaun Predergast | Series 1 Episode 1 | Series 3 Episode 15 |
 | Leanda | Beth Goddard | Series 1 Episode 1 | Series 3 Episode 8 |
 | Dr Dan Acres | Tom Beard | Series 1 Episode 1 | Series 1 Episode 5 |
 | Dr John Reginald | Andrew Ray | Series 1 Episode 1 | Series 3 Episode 13 |
 | Douglas Hart | Maurice Denham | Series 1 Episode 2 | Series 2 Episode 4 |
 | Tony Preston (i) | Adam Berman | Series 1 Episode 3 | Series 4 Episode 2 |
 | Tony Preston (ii) | Adam Croasdall | Series 12 Episode 1 | Series 12 Episode 2 |
 | Julian Preston | Laurie Bilson | Series 1 Episode 3 | Series 4 Episode 2 |
 | Dr Rhiann Lewis | Jaye Griffiths | Series 1 Episode 5 | Series 1 Episode 6 |
 | Rita Barrat | Anne Reid | Series 1 Episode 7 | Series 8 Episode 13 |
 | Ken Alton | Frank Windsor | Series 2 Episode 4 | Series 2 Episode 11 |
 | Laura Elliot | Veronica Roberts | Series 3 Episode 1 | Series 6 Episode 11 |
 | Francine Sinclair | Veronica Quilligin | Series 3 Episode 4 | Series 3 Episode 6 |
 | Janey Cooper | Sasha Paul | Series 3 Episode | Series 3 Episode 7 |
 | Dr Andrew Attwood | Gary Mavers | Series 3 Episode 10 | Series 10 Episode 7 |
 | Dr Erica Matthews | Saskia Wickham | Series 4 Episode 1 | Series 6 Episode 14 |
 | Dr Kate Webster/Preston | Shelagh Mcleod | Series 4 Episode 1 | Series 12 Episode 12 |
 | Russ Skinner | Richard Kerr | Series 4 Episode 1 | Series 4 Episode 10 |
 | Kirsty Attwood | Susie Smith | Series 4 Episode 1 | Series 5 Episode 13 |
 | Gary Sims | Martin Clark | Series 4 Episode 1 | Series 6 Episode 14 |
 | Lee Sims | Dickon Tolson | Series 4 Episode 4 | Series 6 Episode 14 |
 | Charlie Webster (i) | Tim Smith | Series 4 Episode | Series 5 Episode |
 | Charlie Webster (ii) | Chris Trenfield | Series 11 Episode 1 | Series 11 Episode 13 |
 | Charlie Webster (iii) | Stuart Wells | Series 12 Episode 1 | Series 12 Episode 12 |
 | Dr David Shearer | Adrian Lukas | Series 5 Episode 1 | Series 7 Episode 6 |
 | Joanne Pearson | Joy Brook | Series 5 Episode 1 | Series 6 Episode 5 |
 | Claire Shearer (i) | Yolanda Vazquez | Series 5 Episode 3 | Series 5 Episode 14 |
 | Claire Shearer (ii) | Fiona Gillies | Series 6 Episode 1 | Series 7 Episode 13 |
 | Emma Shearer | Jenni Gallagher | Series 5 Episode 3 | Series 7 Episode 7 |
 | Tom Shearer | Nicholas Harvey | Series 5 Episode 3 | Series 7 Episode 7 |
 | Dawn Rudge | Sarah Parish | Series 5 Episode 4 | Series 8 Episode 13 |
 | Trent Rudge | Marc Jordan | Series 6 Episode 1 | Series 8 Episode 13 |
 | Lulu Rudge | Keeley Johnson | Series 6 Episode 1 | Series 8 Episode 13 |
 | Keith Rudge | Gilly Gilchrist | Series 6 Episode 2 | Series 8 Episode 13 |
 | Norman Shorthose | Clive Swift | Series 6 Episode 1 | Series 6 Episode 14 |
 | Polly Stevens | Ludmilla Vuli | Series 6 Episode 1 | Series 6 Episode 14 |
 | Nick Goodson | Thomas Lockyear | Series 6 Episode 5 | Series 6 Episode 8 |
 | Rose Attwood | Barbara Ewing | Series 6 Episode 5 | Series 6 Episode 14 |
 | Chrissy Booth | Kate Fitzgerald | Series 6 Episode 5 | Series 6 Episode 14 |
 | Dr Joanna Graham | Haydn Gwynne | Series 7 Episode 1 | Series 9 Episode 13 |
 | Dr Martin Henderson | Christian Burgess | Series 7 Episode 1 | Series 8 Episode 10 |
 | Ronnie Neale | Rupert Proctor | Series 7 Episode 1 | Series 8 Episode 13 |
 | Georgy Kelper | Jane Slavin | Series 7 Episode 1 | Series 7 Episode 10 |
 | Patricia Davey | Annette Ekblom | Series 7 Episode 2 | Series 7 Episode 7 |
 | Nikki Davey | Georgia Moffett | Series 7 Episode 2 | Series 7 Episode 7 |
 | Bridget Mellors | Siobhan O’Carrol | Series 7 Episode 2 | Series 11 Episode 13 |
 | Liam MacGregor | Paul Blackthorne | Series 7 Episode 2 | Series 7 Episode 7 |
 | Dr Sam Morgan | Joseph Milson | Series 7 Episode 8 | Series 11 Episode 4 |
 | Kelly | Jill Halfpenny | Series 8 Episode 4 | Series 8 Episode 13 |
 | Carol Johnson | Deborah Grant | Series 9 Episode 1 | Series 12 Episode 10 |
 | Richard Turner | David Mallinson | Series 9 Episode 1 | Series 10 Episode 10 |
 | Mike Pullen | John Bowler | Series 9 Episode 2 | Series 11 Episode 13 |
 | Liz Pullen | Melanie Kilburn | Series 9 Episode 2 | Series 9 Episode |
 | Liz Pullen | Regina Freedman | Series 10 Episode 1 | Series 11 Episode 13 |
 | Julie Pullen | Julia Hayworth | Series 9 Episode 2 | Series 11 Episode 13 |
 | Nick Pullen | Karl Davis | Series 9 Episode 2 | Series 11 Episode 13 |
 | Kerri Davidson | Susannah Corbett | Series 9 Episode 2 | Series 12 Episode 13 |
 | Dr Tom Deneley | Gray O’Brien | Series 9 Episode 2 | Series 12 Episode 13 |
 | Serena | Aimee Bracken | Series 9 Episode 4 | Series 9 Episode 9 |
 | Kate Turner | Lynsey Baxter | Series 9 Episode 5 | Series 10 Episode 7 |
 | Lucy Turner | Catherine Holman | Series 9 Episode 5 | Series 10 Episode 7 |
 | Jenny | Denise Black | Series 10 Episode 1 | Series 10 Episode 12 |
 | John Davidson | Russell Dixon | Series 10 Episode 1 | Series 11 Episode 4 |
 | Sylvia Davidson | Romy Baskerville | Series 10 Episode 1 | Series 11 Episode 4 |
 | Dr Alex Redman | Maggie O’Neil | Series 10 Episode 1 | Series 12 Episode 13 |
 | Leanne Shackleton | Christabel Fellowes | Series 10 Episode 4 | Series 12 Episode 13 |
 | Ted Beaumont | Terence Harvey | Series 10 Episode 6 | Series 11 Episode 11 |
 | Dr Mark Kershaw | Paul Opacic | Series 11 Episode 2 | Series 11 Episode 5 |
 | Peter Osbourne | Frank Windsor | Series 11 Episode 10 | Series 11 Episode 11 |
 | Claire Brightwell | Eva Pope | Series 12 Episode 1 | Series 12 Episode 13 |
 | Shaun Carter | Victor McGuire | Series 12 Episode 2 | Series 12 Episode 13 |
 | Dr Matt Kendal | Jamie Bamber | Series 12 Episode 5 | Series 12 Episode 13 |
 | James Strickland | Sean Chapman | Series 12 Episode 5 | Series 12 Episode 12 |
